CADEE: Computer-Aided Directed Evolution of Enzymes

Beat Anton Amrein; Fabian Steffen-Munsberg; Ireneusz Szeler; Miha Purg; Yashraj Kulkarni; Shina Caroline Lynn Kamerlin

doi:10.1107/S2052252516018017

CADEE: Computer-Aided Directed Evolution of Enzymes

Beat Anton Amrein, Fabian Steffen-Munsberg, Ireneusz Szeler, Miha Purg, Yashraj Kulkarni, Shina Caroline Lynn Kamerlin

Uppsala universitet

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of the application of CADEE to a real biological system is also presented in order to illustrate the CADEE workflow.

Originalspråk	engelska
Sidor (från-till)	50-64
Antal sidor	15
Tidskrift	IUCrJ
Volym	4
Nummer	Pt 1
DOI	https://doi.org/10.1107/S2052252516018017
Status	Published - 2017 jan. 1
Externt publicerad	Ja

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abstract = "The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of the application of CADEE to a real biological system is also presented in order to illustrate the CADEE workflow.",

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AU - Kamerlin, Shina Caroline Lynn

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CADEE: Computer-Aided Directed Evolution of Enzymes

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