Identification and characterization of CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase in IgA1-producing cells

Milan Raska, Zina Moldoveanu, Hitoshi Suzuki, Rhubell Brown, Rose Kulhavy, Judit Andrasi, Stacy Hall, Huong L Vu, Fredric Carlsson, Gunnar Lindahl, Milan Tomana, Bruce A Julian, Robert J Wyatt, Jiri Mestecky, Jan Novak

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

39 Citeringar (SciVal)

Sammanfattning

Glycosylation defects occur in several human diseases. In IgA nephropathy, IgA1 contains O-glycans that are galactose-deficient and consist mostly of core 1 alpha2,6 sialylated N-acetylgalactosamine, a configuration suspected to prevent beta1,3 galactosylation. We confirmed the same aberrancy in IgA1 secreted by the human DAKIKI B cell line. Biochemical assays indicated CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase activity in this cell line. However, a candidate enzyme, ST6-GalNAcI, was not transcribed in DAKIKI cells, B cells isolated from blood, or Epstein-Barr virus (EBV)-immortalized IgA1-producing cells from the blood of IgAN patients and healthy controls. Instead, ST6-GalNAcII transcription was detected at a high level. Expression of the ST6-GalNAcII gene and activity of the CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase were higher in IgA1-producing cell lines from IgAN patients than in such cells from healthy controls. These data are the first evidence that human cells that lack ST6-GalNAcI can sialylate core 1 GalNAc-Ser/Thr.
Originalspråkengelska
Sidor (från-till)69-78
TidskriftJournal of Molecular Biology
Volym369
Utgåva1
DOI
StatusPublished - 2007

Ämnesklassifikation (UKÄ)

  • Mikrobiologi inom det medicinska området

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