Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

Katarzyna M. Luda; Clement Da Silva; Fatemeh Ahmadi; Allan Mcl Mowat; Hiroshi Ohno; Knut Kotarsky; William W. Agace

doi:10.1111/sji.13219

Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

Katarzyna M. Luda, Clement Da Silva, Fatemeh Ahmadi, Allan Mcl Mowat, Hiroshi Ohno, Knut Kotarsky, William W. Agace

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103⁺CD11b⁺ cDC in the murine small intestine (SI). GP2 expression was induced on CD103⁺CD11b⁺ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103⁺CD11b⁺ cDC (huLang-Cre.gp2^fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103⁺CD11b⁺ cDC is not required for intestinal immune homoeostasis.

Originalspråk	engelska
Artikelnummer	e13219
Sidor (från-till)	1-12
Tidskrift	Scandinavian Journal of Immunology
Volym	96
Nummer	5
DOI	https://doi.org/10.1111/sji.13219
Status	Published - 2022 nov.

Bibliografisk information

Publisher Copyright:
© 2022 The Authors. Scandinavian Journal of Immunology published by John Wiley & Sons Ltd on behalf of The Scandinavian Foundation for Immunology.

Ämnesklassifikation (UKÄ)

Immunologi inom det medicinska området

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10.1111/sji.13219Licens: CC BY

1 Doktorsavhandling (sammanläggning)

Functional analysis of intestinal dendritic cells
Ahmadi, F., 2023, Lund: Lund University, Faculty of Medicine. 79 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

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@article{859dfe8c562a45e0910299b10ce3e3f4,

title = "Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells",

abstract = "The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.",

keywords = "cDC2, dendritic cell, homeostasis, intestine, IRF4, retinoic acid, TGFβR1",

author = "Luda, {Katarzyna M.} and {Da Silva}, Clement and Fatemeh Ahmadi and Mowat, {Allan Mcl} and Hiroshi Ohno and Knut Kotarsky and Agace, {William W.}",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors. Scandinavian Journal of Immunology published by John Wiley & Sons Ltd on behalf of The Scandinavian Foundation for Immunology.",

year = "2022",

month = nov,

doi = "10.1111/sji.13219",

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T1 - Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

AU - Luda, Katarzyna M.

AU - Da Silva, Clement

AU - Ahmadi, Fatemeh

AU - Mowat, Allan Mcl

AU - Ohno, Hiroshi

AU - Kotarsky, Knut

AU - Agace, William W.

PY - 2022/11

Y1 - 2022/11

N2 - The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.

AB - The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.

KW - cDC2

KW - dendritic cell

KW - homeostasis

KW - intestine

KW - IRF4

KW - retinoic acid

KW - TGFβR1

U2 - 10.1111/sji.13219

DO - 10.1111/sji.13219

M3 - Article

AN - SCOPUS:85140009640

SN - 0300-9475

VL - 96

SP - 1

EP - 12

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

IS - 5

M1 - e13219

ER -

Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

Sammanfattning

Bibliografisk information

Ämnesklassifikation (UKÄ)

Tillgång till dokument

Fingeravtryck

Forskningsoutput

Functional analysis of intestinal dendritic cells

Citera det här