Identification, isolation and analysis of human gut-associated lymphoid tissues

Peter B. Jørgensen, Thomas M. Fenton, Urs M. Mörbe, Lene B. Riis, Henrik L. Jakobsen, Ole H. Nielsen, William W. Agace

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

9 Citeringar (SciVal)

Sammanfattning

Gut-associated lymphoid tissues (GALTs) comprise key intestinal immune inductive sites, including the Peyer’s patches of the small intestine and different types of isolated lymphoid follicle (ILF) found along the length of the gut. Our understanding of human GALT is limited due to a lack of protocols for their isolation. Here we describe a technique that, uniquely among intestinal cell isolation protocols, allows identification and isolation of all human GALT, as well as GALT-free intestinal lamina propria (LP). The technique involves the mechanical separation of intestinal mucosa from the submucosa, allowing the identification and isolation of submucosal ILF (SM-ILF), LP-embedded mucosal ILF (M-ILF) and LP free of contaminating lymphoid tissue. Individual SM-ILF, M-ILF and Peyer’s patch follicles can be subsequently digested for downstream cellular and molecular characterization. The technique, which takes 4–10 h, will be useful for researchers interested in intestinal immune development and function in health and disease.

Originalspråkengelska
Sidor (från-till)2051–2067
Antal sidor17
TidskriftNature Protocols
Volym16
Utgåva4
Tidigt onlinedatum2021 feb. 22
DOI
StatusPublished - 2021 apr. 1

Ämnesklassifikation (UKÄ)

  • Immunologi inom det medicinska området

Fingeravtryck

Utforska forskningsämnen för ”Identification, isolation and analysis of human gut-associated lymphoid tissues”. Tillsammans bildar de ett unikt fingeravtryck.

Citera det här