TY - JOUR
T1 - Immobilisation of lipases by adsorption and deposition
T2 - High protein loading gives lower water activity optimum
AU - Persson, Mattias
AU - Wehtje, Ernst
AU - Adlercreutz, Patrick
PY - 2000/11/27
Y1 - 2000/11/27
N2 - Two different immobilisation techniques for lipases were investigated: adsorption on to Accurel EP-100 and deposition on to Celite. The specific activities were in the same order of magnitude, 2.9 (μmol min -1 mg protein) when Celite was used as support and 2.3 (μmol min -1 mg -1 protein) when Accurel EP-100 was used as support, even if the amount of lipase loaded differed by 2 orders of magnitude. Immobilisation on Accurel EP-100 was the preferred technique since 40-100 times more protein can be loaded/per g carrier, thus yielding a more active catalyst. The water activity profiles in lipase catalysed esterification were influenced by the amount of protein adsorbed to Accurel EP-100. Higher protein loading (40 mg g -1) resulted in a bell-shaped water activity profile with highest specific activity (6.1 μmol min -1 mg -1 protein) at a(w) = 0.11, while an enzyme preparation with low protein loading (4 mg g -1) showed highest specific activity at a(w) = 0.75.
AB - Two different immobilisation techniques for lipases were investigated: adsorption on to Accurel EP-100 and deposition on to Celite. The specific activities were in the same order of magnitude, 2.9 (μmol min -1 mg protein) when Celite was used as support and 2.3 (μmol min -1 mg -1 protein) when Accurel EP-100 was used as support, even if the amount of lipase loaded differed by 2 orders of magnitude. Immobilisation on Accurel EP-100 was the preferred technique since 40-100 times more protein can be loaded/per g carrier, thus yielding a more active catalyst. The water activity profiles in lipase catalysed esterification were influenced by the amount of protein adsorbed to Accurel EP-100. Higher protein loading (40 mg g -1) resulted in a bell-shaped water activity profile with highest specific activity (6.1 μmol min -1 mg -1 protein) at a(w) = 0.11, while an enzyme preparation with low protein loading (4 mg g -1) showed highest specific activity at a(w) = 0.75.
KW - Immobilisation
KW - Lipase
KW - Protein loading
KW - Water activity
UR - http://www.scopus.com/inward/record.url?scp=0033742181&partnerID=8YFLogxK
U2 - 10.1023/A:1005689002238
DO - 10.1023/A:1005689002238
M3 - Article
AN - SCOPUS:0033742181
SN - 0141-5492
VL - 22
SP - 1571
EP - 1575
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 19
ER -