We will present an approach that is based on novel microfluidic devices, utilizing their benefits in processing small-volume samples. Our biomarker discovery strategy, built around this platform, involves optimized samples processing (based on solid-phase extraction and sample enrichment) and fast MALDI-MS read-out. The identification of novel biomarkers at low abundance level has been achieved by the utilization of a miniaturized sample handling platform, which offers clean up and enrichment of proteins in one step. Complete automation has been realized in the form of a unique robotic instrumentation that is able to extract and transfer 96 samples onto standard MALDI target plates with high throughput.
The developed platform was operated with a 60 sample turnaround per hour allowing sensitivities in femtomol regions of medium and low abundant target proteins from clinical studies on tissue samples of multiple sclerosis and gastroesophageal reflux disease. Several proteins have been identified as new biomarkers from cerebrospinal fluid and esophagus epithelial cells.
Bibliografisk informationThe information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Machine Design (011027000), Biomedical Engineering (011200011)
- Analytisk kemi