Increased C-Telopeptide Cross-linking of Tendon Type I Collagen in Fibromodulin-deficient Mice.

Sebastian Kalamajski, Cuiping Liu, Viveka Tillgren, Kristofer Rubin, Åke Oldberg, Jyoti Rai, MaryAnn Weis, David R Eyre

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

45 Citeringar (SciVal)
119 Nedladdningar (Pure)

Sammanfattning

The controlled assembly of collagen monomers into fibrils, with accompanying intermolecular cross-linking by lysyl oxidase-mediated bonds, is vital to the structural and mechanical integrity of connective tissues. This process is influenced by collagen-associated proteins, including Small Leucine-Rich Proteins (SLRPs), but the regulatory mechanisms are not well understood. Deficiency in fibromodulin, an SLRP, causes abnormal collagen fibril ultrastructure and decreased mechanical strength in mouse tendons. In this study, fibromodulin deficiency rendered tendon collagen more resistant to non-proteolytic extraction. The collagen had an increased and altered cross-linking pattern at an early stage of fibril formation. Collagen extracts contained a higher proportion of stably cross-linked α1(I) chains as a result of their C-telopeptide lysines being more completely oxidized to aldehydes. The findings suggest that fibromodulin selectively affects the extent and pattern of lysyl oxidase-mediated collagen cross-linking by sterically hindering access of the enzyme to telopeptides, presumably through binding to the collagen. Such activity implies a broader role for SLRP family members in regulating collagen cross-linking placement and quantity.
Originalspråkengelska
Sidor (från-till)18873-18879
TidskriftJournal of Biological Chemistry
Volym289
Utgåva27
DOI
StatusPublished - 2014

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Connective Tissue Biology (013230151), Åke Oldberg´s group (013212049), Department of Experimental Medical Science (013210000)

Ämnesklassifikation (UKÄ)

  • Cell- och molekylärbiologi

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