Influx and efflux of potassium rubidium-86 in UV-C irradiated guard of Vicia-Faba.

Effects of ultraviolet radiation (UV) (254 nm) on isolated guard cells of Vicia faba (cv. Long pod) have been investigated with regards to fluxes of 86Rb+. UV treatment inhibited influx by about 80%, while it increased efflux by up to 350%. Compartmental analysis showed a highly significant difference (p .ltoreq. 0.005) between the slow leakage components of the UV-treated cells (t1/2 = 5.8 min) and control cells (t1/2 = 72 min). The investigation revealed that the tonoplast (as well as the plasmalemma) is affected by UV.