Instantaneous isotropic volumetric imaging of fast biological processes

Nils Wagner, Nils Norlin, Jakob Gierten, Gustavo de Medeiros, Bálint Balázs, Joachim Wittbrodt, Lars Hufnagel, Robert Prevedel

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

36 Citeringar (SciVal)

Sammanfattning

To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.

Originalspråkengelska
Sidor (från-till)497-500
Antal sidor4
TidskriftNature Methods
Volym16
Utgåva6
DOI
StatusPublished - 2019 jun
Externt publiceradJa

Ämnesklassifikation (UKÄ)

  • Cell- och molekylärbiologi

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