Integrated enzymatic reactions and analysis

Ernst Wehtje; Patrick Adlercreutz; Bo Mattiasson

doi:10.1007/BF02438825

Integrated enzymatic reactions and analysis

Ernst Wehtje, Patrick Adlercreutz, Bo Mattiasson

Bioteknik

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

Enzymatic reactions were performed in a modified auto-injector unit of a Shimadzu HPLC system. The reactions were analyzed by automated injections directly into the HPLC separation system. Two reactions were studied, and the enzymes mandelonitrile lyase and α-chymotrypsin were immobilized by adsorption onto a solid support, e.g., Celite and Chromosorb. The reactions were performed in various organic solvents e.g., diisopropyl ether, heptane/ethyl acetate mixtures and acetonitrile.

Originalspråk	engelska
Sidor (från-till)	165-168
Antal sidor	4
Tidskrift	Biotechnology Techniques
Volym	6
Nummer	2
DOI	https://doi.org/10.1007/BF02438825
Status	Published - 1992 mars 1

Ämnesklassifikation (UKÄ)

Biokatalys och enzymteknik

Tillgång till dokument

10.1007/BF02438825

Andra filer och länkar

Link to publication in Scopus

Citera det här

@article{1055c566d4f5480f9c770f98f2633e09,

title = "Integrated enzymatic reactions and analysis",

abstract = "Enzymatic reactions were performed in a modified auto-injector unit of a Shimadzu HPLC system. The reactions were analyzed by automated injections directly into the HPLC separation system. Two reactions were studied, and the enzymes mandelonitrile lyase and α-chymotrypsin were immobilized by adsorption onto a solid support, e.g., Celite and Chromosorb. The reactions were performed in various organic solvents e.g., diisopropyl ether, heptane/ethyl acetate mixtures and acetonitrile.",

author = "Ernst Wehtje and Patrick Adlercreutz and Bo Mattiasson",

year = "1992",

month = mar,

day = "1",

doi = "10.1007/BF02438825",

language = "English",

volume = "6",

pages = "165--168",

journal = "Biotechnology Techniques",

issn = "0951-208X",

publisher = "Springer",

number = "2",

}

TY - JOUR

T1 - Integrated enzymatic reactions and analysis

AU - Wehtje, Ernst

AU - Adlercreutz, Patrick

AU - Mattiasson, Bo

PY - 1992/3/1

Y1 - 1992/3/1

N2 - Enzymatic reactions were performed in a modified auto-injector unit of a Shimadzu HPLC system. The reactions were analyzed by automated injections directly into the HPLC separation system. Two reactions were studied, and the enzymes mandelonitrile lyase and α-chymotrypsin were immobilized by adsorption onto a solid support, e.g., Celite and Chromosorb. The reactions were performed in various organic solvents e.g., diisopropyl ether, heptane/ethyl acetate mixtures and acetonitrile.

AB - Enzymatic reactions were performed in a modified auto-injector unit of a Shimadzu HPLC system. The reactions were analyzed by automated injections directly into the HPLC separation system. Two reactions were studied, and the enzymes mandelonitrile lyase and α-chymotrypsin were immobilized by adsorption onto a solid support, e.g., Celite and Chromosorb. The reactions were performed in various organic solvents e.g., diisopropyl ether, heptane/ethyl acetate mixtures and acetonitrile.

UR - http://www.scopus.com/inward/record.url?scp=51649132613&partnerID=8YFLogxK

U2 - 10.1007/BF02438825

DO - 10.1007/BF02438825

M3 - Article

AN - SCOPUS:51649132613

SN - 0951-208X

VL - 6

SP - 165

EP - 168

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 2

ER -

Integrated enzymatic reactions and analysis

Sammanfattning

Ämnesklassifikation (UKÄ)

Tillgång till dokument

Andra filer och länkar

Fingeravtryck

Citera det här