Isolation and Purification of Mycobacterial Extracellular Vesicles

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Sammanfattning

Bacterial extracellular vesicles (EVs) contain numerous active substances that mediate bacterial interactions with their host and with other microbes. Best defined are the EVs from Gram-negative bacteria that have been shown to deliver virulence factors, modulate the immune responses, mediate antibiotic resistance, and also inhibit competitive microbes. Due to the complex cell wall structures of Gram-positive bacteria and mycobacteria, EVs from these bacteria were only recently reported. This protocol describes the isolation of EVs from mycobacteria.
Originalspråksvenska
Titel på värdpublikationBacterial Pathogenesis
RedaktörerPontus Nordenfelt, Mattias Collin
UtgivningsortNew York, NY, USA
FörlagSpringer
Kapitel3
Sidor55-60
Volym2674
Utgåva2nd
ISBN (elektroniskt)978-1-0716-3243-7
ISBN (tryckt)978-1-0716-3242-0
DOI
StatusPublished - 2023

Publikationsserier

NamnMethods in molecular biology
Volym2674
ISSN (tryckt)1064-3745
ISSN (elektroniskt)1940-6029

Ämnesklassifikation (UKÄ)

  • Mikrobiologi inom det medicinska området

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