Isolation of human complement factors C3, C5 and H

Åke Lundwall, G. Eggertsen

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

An improved method for simultaneous purification of complement factors C3, C5 and H from human plasma has been developed. Using an initial batch separation technique with QAE-Sephadex, followed by chromatography on SP-Sephadex and gel filtration in Sephadex G-200, 600 mg of highly pure C3 can be prepared from 1600 ml of plasma. Simultaneously about 70 mg of highly pure factor H and 30 mg of C5 are obtained by chromatography of post SP-Sephadex material on DEAE-Sephacel. A small amount of C3 in the C5 pool is removed by anti-C3-Sepharose. By maleylation or citraconylation of reduced and alkylated C3, the constitutive polypeptide chains are modified in a way that made them separable by ion exchange chromatography.
Originalspråkengelska
Sidor (från-till)147-60
TidskriftJournal of Immunological Methods
Volym81
Nummer1
StatusPublished - 1985
Externt publiceradJa

Bibliografisk information

1

Ämnesklassifikation (UKÄ)

  • Läkemedelskemi

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