Sammanfattning
An improved method for simultaneous purification of complement factors C3, C5 and H from human plasma has been developed. Using an initial batch separation technique with QAE-Sephadex, followed by chromatography on SP-Sephadex and gel filtration in Sephadex G-200, 600 mg of highly pure C3 can be prepared from 1600 ml of plasma. Simultaneously about 70 mg of highly pure factor H and 30 mg of C5 are obtained by chromatography of post SP-Sephadex material on DEAE-Sephacel. A small amount of C3 in the C5 pool is removed by anti-C3-Sepharose. By maleylation or citraconylation of reduced and alkylated C3, the constitutive polypeptide chains are modified in a way that made them separable by ion exchange chromatography.
Originalspråk | engelska |
---|---|
Sidor (från-till) | 147-60 |
Tidskrift | Journal of Immunological Methods |
Volym | 81 |
Nummer | 1 |
Status | Published - 1985 |
Externt publicerad | Ja |
Bibliografisk information
1Ämnesklassifikation (UKÄ)
- Läkemedelskemi