TRPA5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect

Marjorie A. Liénard, David Baez-Nieto, Cheng-Chia Tsai, Wendy A. Valencia-Montoya, Balder Werin, Urban Johanson, Jean-Marc Lassance, Jen Q. Pan, Nanfang Yu, Naomi E. Pierce

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

As ectotherms, insects need heat-sensitive receptors to monitor environmental temperatures and facilitate thermoregulation. We show that TRPA5, a class of ankyrin transient receptor potential (TRP) channels absent in dipteran genomes, may function as insect heat receptors. In the triatomine bug Rhodnius prolixus (order: Hemiptera), a vector of Chagas disease, the channel RpTRPA5B displays a uniquely high thermosensitivity, with biophysical determinants including a large channel activation enthalpy change (72 kcal/mol), a high temperature coefficient (Q10 = 25), and in vitro temperature-induced currents from 53°C to 68°C (T0.5 = 58.6°C), similar to noxious TRPV receptors in mammals. Monomeric and tetrameric ion channel structure predictions show reliable parallels with fruit fly dTRPA1, with structural uniqueness in ankyrin repeat domains, the channel selectivity filter, and potential TRP functional modulator regions. Overall, the finding of a member of TRPA5 as a temperature-activated receptor illustrates the diversity of insect molecular heat detectors.
Originalspråkengelska
Artikelnummer109541
Antal sidor25
TidskriftiScience
Volym27
Nummer4
DOI
StatusPublished - 2024 apr. 19

Bibliografisk information

© 2024 The Author(s).

Ämnesklassifikation (UKÄ)

  • Zoologi
  • Biokemi och molekylärbiologi

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