Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source

Filip Leonarski, Jie Nan, Zdenek Matej, Quentin Bertrand, Antonia Furrer, Ishkhan Gorgisyan, Monika Bjelčić, Michal Kepa, Hannah Glover, Viktoria Hinger, Thomas Eriksson, Aleksander Cehovin, Mikel Eguiraun, Piero Gasparotto, Aldo Mozzanica, Tobias Weinert, Ana Gonzalez, Jörg Standfuss, Meitian Wang, Thomas UrsbyFlorian Dworkowski

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most thirdgeneration synchrotron-based macromolecular crystallography beamlines do not offer the necessary infrastructure yet. Here, a new setup is demonstrated, based on the JUNGFRAU detector and Jungfraujoch data-acquisition system, that enables collection of kilohertz serial crystallography data at fourthgeneration synchrotrons. More importantly, it is shown that this setup is capable of collecting multiple-time-point time-resolved protein dynamics at kilohertz rates, allowing the probing of microsecond to second dynamics at synchrotrons in a fraction of the time needed previously. A high-quality complete X-ray dataset was obtained within 1 min from lysozyme microcrystals, and the dynamics of the light-driven sodium-pump membrane protein KR2 with a time resolution of 1 ms could be demonstrated. To make the setup more accessible for researchers, downstream data handling and analysis will be automated to allow on-the-fly spot finding and indexing, as well as data processing.

Originalspråkengelska
Sidor (från-till)729-737
Antal sidor9
TidskriftIUCrJ
Volym10
NummerPt 6
DOI
StatusPublished - 2023 okt. 14

Ämnesklassifikation (UKÄ)

  • Atom- och molekylfysik och optik

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