Large-scale inference of protein tissue origin in gram-positive sepsis plasma using quantitative targeted proteomics.

Erik Malmström, Ola Kilsgård, Simon Hauri, Emanuel Smeds, Heiko Herwald, Lars Malmström, Johan Malmström

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

335 Nedladdningar (Pure)

Sammanfattning

The plasma proteome is highly dynamic and variable, composed of proteins derived from surrounding tissues and cells. To investigate the complex processes that control the composition of the plasma proteome, we developed a mass spectrometry-based proteomics strategy to infer the origin of proteins detected in murine plasma. The strategy relies on the construction of a comprehensive protein tissue atlas from cells and highly vascularized organs using shotgun mass spectrometry. The protein tissue atlas was transformed to a spectral library for highly reproducible quantification of tissue-specific proteins directly in plasma using SWATH-like data-independent mass spectrometry analysis. We show that the method can determine drastic changes of tissue-specific protein profiles in blood plasma from mouse animal models with sepsis. The strategy can be extended to several other species advancing our understanding of the complex processes that contribute to the plasma proteome dynamics.
Originalspråkengelska
Artikelnummer10261
TidskriftNature Communications
Volym7
DOI
StatusPublished - 2016

Ämnesklassifikation (UKÄ)

  • Cell- och molekylärbiologi

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