TY - JOUR
T1 - Lipase-catalyzed alcoholysis of triglycerides for the preparation of 2-monoglycerides
AU - Millqvist, Anna
AU - Adlercreutz, Patrick
AU - Mattiasson, Bo
PY - 1994/1/1
Y1 - 1994/1/1
N2 - Rhizopus arrhizus lipase immobilized on celite was used to prepare isomerically pure 2-monoglycerides by alcoholysis of triglycerides in organic media. Reaction parameters such as choice of solvent, choice of alcohol, and alcohol concentration were studied. When 12.5 mM tripalmitin was used as substrate, methyl-tert-butyl ether was the best solvent for alcoholysis at water activity 0.11. Ethanol gave the highest yield (97%) at an optimal ethanol concentration of 200-300 mM. At higher alcohol concentrations, the enzyme activity was substantially lowered. The enzyme preparation showed high stability in repeated-batch reactions.
AB - Rhizopus arrhizus lipase immobilized on celite was used to prepare isomerically pure 2-monoglycerides by alcoholysis of triglycerides in organic media. Reaction parameters such as choice of solvent, choice of alcohol, and alcohol concentration were studied. When 12.5 mM tripalmitin was used as substrate, methyl-tert-butyl ether was the best solvent for alcoholysis at water activity 0.11. Ethanol gave the highest yield (97%) at an optimal ethanol concentration of 200-300 mM. At higher alcohol concentrations, the enzyme activity was substantially lowered. The enzyme preparation showed high stability in repeated-batch reactions.
KW - 2-monoglycerides
KW - alcoholysis
KW - hydrolysis
KW - Lipase
KW - operational stability
KW - organic solvents
KW - water activity
UR - http://www.scopus.com/inward/record.url?scp=0028657428&partnerID=8YFLogxK
U2 - 10.1016/0141-0229(94)90140-6
DO - 10.1016/0141-0229(94)90140-6
M3 - Article
AN - SCOPUS:0028657428
SN - 0141-0229
VL - 16
SP - 1042
EP - 1047
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 12
ER -