Massive intein content in Anaeramoeba reveals aspects of intein mobility in eukaryotes

Lucie Gallot-Lavallée, Jon Jerlström-Hultqvist, Paula Zegarra-Vidarte, Dayana E Salas-Leiva, Courtney W Stairs, Ivan Čepička, Andrew J Roger, John M Archibald

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review


Inteins are self-splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here, we show that the nuclear genomes of three strains of Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryotic genome described previously. The Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein-containing proteins in other eukaryotes, prokaryotes, and viruses. Our data also suggest that viruses have contributed to the spread of inteins in Anaeramoeba and the colonization of new alleles. The persistence of Anaeramoeba inteins might be partly explained by intragenomic movement of intein-encoding regions from gene to gene. Our intein dataset greatly expands the spectrum of intein-containing proteins and provides insights into the evolution of inteins in eukaryotes.

Antal sidor9
TidskriftProceedings of the National Academy of Sciences of the United States of America
StatusPublished - 2023 dec. 5

Ämnesklassifikation (UKÄ)

  • Mikrobiologi
  • Genetik


Utforska forskningsämnen för ”Massive intein content in Anaeramoeba reveals aspects of intein mobility in eukaryotes”. Tillsammans bildar de ett unikt fingeravtryck.

Citera det här