Molecular engineering of a thermostable carbohydrate-binding module

Lavinia Cicortas Gunnarsson, Eva Nordberg Karlsson, Mats Andersson, Olle Holst, Mats Ohlin

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKonferenspaper i proceedingPeer review

2 Citeringar (SciVal)

Sammanfattning

Structure-function studies are frequently practiced on the very diverse group of natural carbohydrate-binding modules in order to understand the target recognition of these proteins. We have taken a step further in the study of carbohydrate-binding modules and created variants with novel binding properties by molecular engineering of one such molecule of known 3D-structure. A combinatorial library was created from the sequence encoding a thermostable carbohydrate-binding module, CBM4-2 from a Rhodothermus marinus xylanase, and phage-display technology was successfully used for selection of variants with specificity towards different carbohydrate polymers (birchwood xylan, Avicel (TM), ivory nut mannan and recently also xyloglucan), as well as towards a glycoprotein (human IgG4). Our work not only generated a number of binders with properties that would suite a range of biotechnological applications, but analysis of selected binders also helped us to identify residues important for their specificities.
Originalspråkengelska
Titel på värdpublikationBiocatalysis and Biotransformation
FörlagTaylor & Francis
Sidor31-37
Volym24
DOI
StatusPublished - 2006
EvenemangSixth Carbohydrate Bioengineering Meeting - Barcelona, Spanien
Varaktighet: 2005 apr. 32005 apr. 6

Publikationsserier

Namn
Nummer1-2
Volym24
ISSN (tryckt)1024-2422
ISSN (elektroniskt)1029-2446

Konferens

KonferensSixth Carbohydrate Bioengineering Meeting
Land/TerritoriumSpanien
OrtBarcelona
Period2005/04/032005/04/06

Ämnesklassifikation (UKÄ)

  • Immunologi inom det medicinska området
  • Industriell bioteknik

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