Molecularly imprinted microspheres as antibody binding mimics

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review


Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of functional monomer and crosslinker in the presence of a print molecule. The method is generally applicable to a large variety of target molecules. For the first time, an enzyme probe is successfully used in a competitive herbicide assay using molecularly imprinted microspheres as the affinity adsorbents.

Sidor (från-till)149-157
Antal sidor9
TidskriftReactive and Functional Polymers
StatusPublished - 2001 maj 2
Evenemang9th International Conference on Polymer-Based Technology - , Kina
Varaktighet: 2000 maj 212000 maj 21

Ämnesklassifikation (UKÄ)

  • Annan industriell bioteknik


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