Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.

Laura Muresan, Mihaela Nistor, Szilveszter Gáspár, Ionel Cătălin Popescu, Elisabeth Csöregi

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection.
Originalspråkengelska
Sidor (från-till)81-86
TidskriftBioelectrochemistry
Volym76
DOI
StatusPublished - 2009

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Ämnesklassifikation (UKÄ)

  • Analytisk kemi

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