Monovalent Interactions of Galectin-1

Emma Salomonsson, Amaia Larumbe, Johan Tejler, Erik Tullberg, Hanna Rydberg, Anders Sundin, Areej Khabut, Torbjörn Frejd, Yuri Lobsanov, James M. Rini, Ulf Nilsson, Hakon Leffler

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

50 Citeringar (SciVal)

Sammanfattning

Galectin-1, beta-galactoside binding lectin involved in immunoregulation and cancer, binds natural and many synthetic multivalent glycoconjugates with an apparent glycoside cluster effect, that is, affinity above and beyond what would be expected from the concentration of the determinant sugar. Here we have analyzed the mechanism of such cluster effects in solution at physiological concentration using a fluorescence anisotropy assay with a novel fluorescent high-affinity galectin-1 binding probe. The interaction of native dimeric and monomeric mutants of rat and human galectin-1 with mono- and divalent small molecules, fetuin, asialofetuin, and human serum glycoproteins was analyzed. Surprisingly, high-affinity binding did not depend much on the dimeric state of galectin-1 and thus is due mainly to monomeric interactions of a single carbohydrate recognition domain. The mechanism for this is unknown, but one possibility includes additional interactions that high-affinity ligands make with an extended binding site on the carbohydrate recognition domain, It follows that such weak additional interactions must be important for the biological function of galectin-1 and also for the design of galectin-1 inhibitors.
Originalspråkengelska
Sidor (från-till)9518-9532
TidskriftBiochemistry
Volym49
Utgåva44
DOI
StatusPublished - 2010

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Organic chemistry (S/LTH) (011001240), Division of Microbiology, Immunology and Glycobiology - MIG (013025200), Department of Laboratory Medicine, Lund (013017000)

Ämnesklassifikation (UKÄ)

  • Biokemi och molekylärbiologi

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