NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg–protoporphyrin monomethyl ester cyclase

Anne Stenbaek; Andreas Hansson; Ragna Peterson Wulff; Mats Hansson; Karl-Josef Dietz; Poul Erik Jensen

doi:10.1016/j.febslet.2008.07.006

NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg–protoporphyrin monomethyl ester cyclase

Anne Stenbaek, Andreas Hansson, Ragna Peterson Wulff, Mats Hansson, Karl-Josef Dietz, Poul Erik Jensen

Biokemi och Strukturbiologi

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

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Abstract The chloroplast-localized NADPH-dependent thioredoxin
reductase (NTRC) has been found to be able to reduce
hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show
that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis
and accumulate intermediates preceding protochlorophyllide
formation. A specific involvement of NTRC during
biosynthesis of protochlorophyllide is indicated from in vitro aerobic
cyclase assays in which the conversion of Mg–protoporhyrin
monomethyl ester into protochlorophyllide is stimulated by addition
of the NTRC/2-Cys peroxiredoxin system. These findings
support the hypothesis that this NADPH-dependent hydrogen
peroxide scavenging system is particularly important during
periods with limited reducing power from photosynthesis, e.g. under
chloroplast biogenesis.

Originalspråk	engelska
Sidor (från-till)	2773-2778
Tidskrift	FEBS Letters
Volym	582
Nummer	18
DOI	https://doi.org/10.1016/j.febslet.2008.07.006
Status	Published - 2008

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10.1016/j.febslet.2008.07.006

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title = "NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg–protoporphyrin monomethyl ester cyclase",

abstract = "Abstract The chloroplast-localized NADPH-dependent thioredoxin reductase (NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg–protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e.g. under chloroplast biogenesis.",

keywords = "Gene co-expression, Chloroplast biogenesis, Biosynthetic pathway, Hydrogen peroxide scavenging, Arabidopsis thaliana, Aerobic cyclase",

author = "Anne Stenbaek and Andreas Hansson and {Peterson Wulff}, Ragna and Mats Hansson and Karl-Josef Dietz and Jensen, {Poul Erik}",

year = "2008",

doi = "10.1016/j.febslet.2008.07.006",

language = "English",

volume = "582",

pages = "2773--2778",

journal = "FEBS Letters",

issn = "1873-3468",

publisher = "Wiley-Blackwell",

number = "18",

}

TY - JOUR

T1 - NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg–protoporphyrin monomethyl ester cyclase

AU - Stenbaek, Anne

AU - Hansson, Andreas

AU - Peterson Wulff, Ragna

AU - Hansson, Mats

AU - Dietz, Karl-Josef

AU - Jensen, Poul Erik

PY - 2008

Y1 - 2008

N2 - Abstract The chloroplast-localized NADPH-dependent thioredoxin reductase (NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg–protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e.g. under chloroplast biogenesis.

AB - Abstract The chloroplast-localized NADPH-dependent thioredoxin reductase (NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg–protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e.g. under chloroplast biogenesis.

KW - Gene co-expression

KW - Chloroplast biogenesis

KW - Biosynthetic pathway

KW - Hydrogen peroxide scavenging

KW - Arabidopsis thaliana

KW - Aerobic cyclase

U2 - 10.1016/j.febslet.2008.07.006

DO - 10.1016/j.febslet.2008.07.006

M3 - Article

C2 - 18625226

SN - 1873-3468

VL - 582

SP - 2773

EP - 2778

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -

NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg–protoporphyrin monomethyl ester cyclase

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