Nucleolar proteins with altered expression in leukemic cell lines.

Kaisa J Teittinen; Pauliina Kärkkäinen; Johanna Salonen; Gunilla Rönnholm; Hanna Korkeamäki; Mauno Vihinen; Nisse Kalkkinen; Olli Lohi

doi:10.1016/j.leukres.2011.06.038

Nucleolar proteins with altered expression in leukemic cell lines.

Kaisa J Teittinen, Pauliina Kärkkäinen, Johanna Salonen, Gunilla Rönnholm, Hanna Korkeamäki, Mauno Vihinen, Nisse Kalkkinen, Olli Lohi

University of Tampere

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

The function of the nucleolus is intimately connected to cell proliferation, division and growth. Many cancer cells have enlarged nucleoli, and several nucleolar proteins have been linked to tumorigenesis. In order to find proteins whose expression is altered in the nucleoli of leukemic cells, we carried out two-dimensional difference gel electrophoresis (2-D DIGE) analyses. Prohibitin (PHB) and TAR-DNA-binding protein-43 (TDP-43) were strongly expressed in the nucleoli of the pre-B-ALL cell line MHH-CALL3. Our results demonstrate that leukemic cells have differences in their nucleolar protein composition, and suggest that it may be possible to exploit these differences in identification of leukemia subtypes.

Originalspråk	engelska
Sidor (från-till)	232-236
Tidskrift	Leukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis
Volym	36
Nummer	2
DOI	https://doi.org/10.1016/j.leukres.2011.06.038
Status	Published - 2012
Externt publicerad	Ja

Ämnesklassifikation (UKÄ)

Cancer och onkologi

FN:s Globala mål

Denna forskningsoutput relaterar till följande Globala mål

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10.1016/j.leukres.2011.06.038

http://www.ncbi.nlm.nih.gov/pubmed/21783252?dopt=Abstract

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title = "Nucleolar proteins with altered expression in leukemic cell lines.",

abstract = "The function of the nucleolus is intimately connected to cell proliferation, division and growth. Many cancer cells have enlarged nucleoli, and several nucleolar proteins have been linked to tumorigenesis. In order to find proteins whose expression is altered in the nucleoli of leukemic cells, we carried out two-dimensional difference gel electrophoresis (2-D DIGE) analyses. Prohibitin (PHB) and TAR-DNA-binding protein-43 (TDP-43) were strongly expressed in the nucleoli of the pre-B-ALL cell line MHH-CALL3. Our results demonstrate that leukemic cells have differences in their nucleolar protein composition, and suggest that it may be possible to exploit these differences in identification of leukemia subtypes.",

keywords = "Repressor Proteins: metabolism, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma: metabolism, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma: diagnosis, Nuclear Proteins: metabolism, Cell Nucleolus: metabolism, DNA-Binding Proteins: metabolism, Tumor Markers, Biological: metabolism",

author = "Teittinen, \{Kaisa J\} and Pauliina K{\"a}rkk{\"a}inen and Johanna Salonen and Gunilla R{\"o}nnholm and Hanna Korkeam{\"a}ki and Mauno Vihinen and Nisse Kalkkinen and Olli Lohi",

year = "2012",

doi = "10.1016/j.leukres.2011.06.038",

language = "English",

volume = "36",

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journal = "Leukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis",

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TY - JOUR

T1 - Nucleolar proteins with altered expression in leukemic cell lines.

AU - Teittinen, Kaisa J

AU - Kärkkäinen, Pauliina

AU - Salonen, Johanna

AU - Rönnholm, Gunilla

AU - Korkeamäki, Hanna

AU - Vihinen, Mauno

AU - Kalkkinen, Nisse

AU - Lohi, Olli

PY - 2012

Y1 - 2012

N2 - The function of the nucleolus is intimately connected to cell proliferation, division and growth. Many cancer cells have enlarged nucleoli, and several nucleolar proteins have been linked to tumorigenesis. In order to find proteins whose expression is altered in the nucleoli of leukemic cells, we carried out two-dimensional difference gel electrophoresis (2-D DIGE) analyses. Prohibitin (PHB) and TAR-DNA-binding protein-43 (TDP-43) were strongly expressed in the nucleoli of the pre-B-ALL cell line MHH-CALL3. Our results demonstrate that leukemic cells have differences in their nucleolar protein composition, and suggest that it may be possible to exploit these differences in identification of leukemia subtypes.

AB - The function of the nucleolus is intimately connected to cell proliferation, division and growth. Many cancer cells have enlarged nucleoli, and several nucleolar proteins have been linked to tumorigenesis. In order to find proteins whose expression is altered in the nucleoli of leukemic cells, we carried out two-dimensional difference gel electrophoresis (2-D DIGE) analyses. Prohibitin (PHB) and TAR-DNA-binding protein-43 (TDP-43) were strongly expressed in the nucleoli of the pre-B-ALL cell line MHH-CALL3. Our results demonstrate that leukemic cells have differences in their nucleolar protein composition, and suggest that it may be possible to exploit these differences in identification of leukemia subtypes.

KW - Repressor Proteins: metabolism

KW - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma: metabolism

KW - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma: diagnosis

KW - Nuclear Proteins: metabolism

KW - Cell Nucleolus: metabolism

KW - DNA-Binding Proteins: metabolism

KW - Tumor Markers

KW - Biological: metabolism

UR - https://www.scopus.com/pages/publications/84855194324

U2 - 10.1016/j.leukres.2011.06.038

DO - 10.1016/j.leukres.2011.06.038

M3 - Article

SN - 1873-5835

VL - 36

SP - 232

EP - 236

JO - Leukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis

JF - Leukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis

IS - 2

ER -

Nucleolar proteins with altered expression in leukemic cell lines.

Sammanfattning

Ämnesklassifikation (UKÄ)

FN:s Globala mål

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