Optical projection tomography for rapid whole mouse brain imaging

David Nguyen, Paul J. Marchand, Arielle L. Planchette, Julia Nilsson, Miguel Sison, Jérôme Extermann, Antonio Lopez, Marcin Sylwestrzak, Jessica Sordet-Dessimoz, Anja Schmidt-Christensen, Dan Holmberg, Dimitri Van De Ville, Theo Lasser

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

22 Citeringar (SciVal)

Sammanfattning

In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.

Originalspråkengelska
Artikelnummer#301816
Sidor (från-till)5637-5650
Antal sidor14
TidskriftBiomedical Optics Express
Volym8
Nummer12
DOI
StatusPublished - 2017 dec. 1

Ämnesklassifikation (UKÄ)

  • Radiologi och bildbehandling

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