ParA and ParB coordinate chromosome segregation with cell elongation and division during Streptomyces sporulation

Magdalena Donczew, Paweł Mackiewicz, Agnieszka Wróbel, Klas Flärdh, Jolanta Zakrzewska-Czerwińska, Dagmara Jakimowicz

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

In unicellular bacteria, the ParA and ParB proteins segregate chromosomes and coordinate this process with cell division and chromosome replication. During sporulation of mycelial Streptomyces, ParA and ParB uniformly distribute multiple chromosomes along the filamentous sporogenic hyphal compartment, which then differentiates into a chain of unigenomic spores. However, chromosome segregation must be coordinated with cell elongation and multiple divisions. Here, we addressed the question of whether ParA and ParB are involved in the synchronization of cell-cycle processes during sporulation in Streptomyces. To answer this question, we used time-lapse microscopy, which allows the monitoring of growth and division of single sporogenic hyphae. We showed that sporogenic hyphae stop extending at the time of ParA accumulation and Z-ring formation. We demonstrated that both ParA and ParB affect the rate of hyphal extension. Additionally, we showed that ParA promotes the formation of massive nucleoprotein complexes by ParB.We also showed that FtsZ ring assembly is affected by the ParB protein and/or unsegregated DNA. Our results indicate the existence of a checkpoint between the extension and septation of sporogenic hyphae that involves the ParA and ParB proteins.

Originalspråkengelska
Artikelnummer150263
Antal sidor15
TidskriftOpen biology
Volym6
Nummer4
DOI
StatusPublished - 2016

Bibliografisk information

Publisher Copyright:
© 2016 The Authors.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Ämnesklassifikation (UKÄ)

  • Cellbiologi

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