Passenger strand loading in overexpression experiments using microRNA mimics.

Rolf Sökilde, Inga Newie, Helena Persson, Åke Borg, Carlos Rovira

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

MicroRNAs (miRNAs) are important regulators of gene function and manipulation of miRNAs is a central component of basic research. Modulation of gene expression by miRNA gain-of-function can be based on different approaches including transfection with miRNA mimics; artificial, chemically modified miRNA-like small RNAs. These molecules are intended to mimic the function of a miRNA guide strand while bypassing the maturation steps of endogenous miRNAs. Due to easy accessibility through commercial providers this approach has gained popularity, and accuracy is often assumed without prior independent testing. Our in silico analysis of over-represented sequence motifs in microarray expression data and sequencing of AGO-associated small RNAs indicate, however, that miRNA mimics may be associated with considerable side-effects due to the unwanted activity of the miRNA mimic complementary strand.
Originalspråkengelska
Sidor (från-till)787-791
TidskriftRNA Biology
Volym12
Nummer8
DOI
StatusPublished - 2015

Ämnesklassifikation (UKÄ)

  • Cell- och molekylärbiologi

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