Profiling of internalizing tumor-associated antigens on breast and pancreatic cancer cells by reversed genomics

Johan Fransson, Sara Ek, Peter Ellmark, Eskil Söderlind, Carl Borrebaeck, Christina Furebring

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Human antibodies directed towards functionally associated tumor antigens have great potentials as adjuvant treatment in cancer therapy. Here we describe an efficient subtractive approach to select single chain Fv (scFv) antibodies, specifically binding to unknown rapidly internalizing tumor-associated antigens (TAA) on human breast and pancreatic carcinoma cell lines. After re-engineering the scFv into intact IgG molecules, these fully human antibodies displayed individual binding profiles to TAAs on breast, pancreatic, colorectal and prostate carcinomas, while showing no reactivity to lymphomas. The ability of the selected antibodies to undergo receptor-mediated internalization was verified by confocal microscopy, thus proving our strategy to provide a unique set of human antibodies, potentially useful in immunotherapy. (C) 2003 Elsevier Ltd. All rights reserved.
Originalspråkengelska
Sidor (från-till)235-242
TidskriftCancer Letters
Volym208
Nummer2
DOI
StatusPublished - 2004

Ämnesklassifikation (UKÄ)

  • Cancer och onkologi

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