TY - JOUR
T1 - Protein kinase C inhibition has only a transient growth arresting effect on in vitro regenerating mouse sensory neurons
AU - Wiklund, Peter
AU - Ekström, Per
PY - 1999/11/19
Y1 - 1999/11/19
N2 - Adult mice sensory ganglia were cultured in an extracellular matrix gel. Analyses of extending axons were made 48 h (long-term) or immediately (short-term) after addition of protein kinase inhibitors. Long- and short-term growth was insensitive to protein kinase A/G inhibition by HA-1004. Long-term protein kinase C inhibition by chelerythrine affected only certain, long axons. In the short-term virtually all axon growth was arrested, but largely recovered on the following day. When combined, the drugs inhibited all long- and short-term growth and largely prevented the recovery of the latter. The transient effect by chelerythrine, and the permanent inhibition after combination with HA-1004, suggests compensatory mechanisms, perhaps via other kinases. Copyright (C) 1999 Elsevier Science Ireland Ltd.
AB - Adult mice sensory ganglia were cultured in an extracellular matrix gel. Analyses of extending axons were made 48 h (long-term) or immediately (short-term) after addition of protein kinase inhibitors. Long- and short-term growth was insensitive to protein kinase A/G inhibition by HA-1004. Long-term protein kinase C inhibition by chelerythrine affected only certain, long axons. In the short-term virtually all axon growth was arrested, but largely recovered on the following day. When combined, the drugs inhibited all long- and short-term growth and largely prevented the recovery of the latter. The transient effect by chelerythrine, and the permanent inhibition after combination with HA-1004, suggests compensatory mechanisms, perhaps via other kinases. Copyright (C) 1999 Elsevier Science Ireland Ltd.
KW - Chelerythrine chloride
KW - Dorsal root ganglia
KW - Growth cones
KW - HA-1004
KW - Protein kinase A
KW - Protein kinase G
UR - http://www.scopus.com/inward/record.url?scp=0032754898&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(99)00720-X
DO - 10.1016/S0304-3940(99)00720-X
M3 - Article
C2 - 10580698
AN - SCOPUS:0032754898
SN - 0304-3940
VL - 275
SP - 155
EP - 158
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -