Protein signatures of remodeled airways in transplanted lungs with Bronchiolitis Obliterans Syndrome obtained using laser capture microdissection

Catharina Müller, Oskar Rosmark, Emma Åhrman, Hans Brunnström, Katharina Wassilew, Annika Nybom, Barbora Michaliková, Hillevi Larsson, Leif T Eriksson, Hans Henrik Schultz, Michael Perch, Johan Malmström, Jenny Wigén, Martin Iversen, Gunilla Westergren-Thorsson

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Bronchiolitis obliterans syndrome (BOS), a common form of chronic lung allograft dysfunction, is the major limitation to long-term survival after lung transplantation. The histological correlate is progressive, fibrotic occlusion of small airways, obliterative bronchiolitis lesions, ultimately leading to organ failure. The molecular composition of these lesions is unknown. By laser-capture microdissection and optimized sample preparation protocols for mass spectrometry the protein composition of the lesions in explanted lungs from four end-stage BOS patients were analysed. Immunohistochemistry and immunofluorescence were used to determine the spatial distribution of commonly identified proteins on the tissue level, protein signatures for in total 14 OB-lesions were established. A set of 39 proteins identified in more than 75% of lesions included distinct structural proteins (collagen type IV and VI) and cellular components (actins, vimentin, tryptase). Each respective lesion exhibited a unique composition of proteins (on average n=66 proteins), thereby mirroring the morphological variation of the lesions. Antibody-based staining confirmed these MS-based findings. The 14 analyzed OB-lesions showed variations in their protein content, but also common features. This study provides molecular and morphological insights into the development of chronic rejection after lung transplantation. The protein patterns in the lesions were correlated to pathways of extracellular matrix organization, tissue development and wound healing processes.

Originalspråkengelska
Sidor (från-till)1398-1411
Antal sidor14
TidskriftAmerican Journal of Pathology
Volym191
Utgåva8
Tidigt onlinedatum2021 juni 7
DOI
StatusPublished - 2021 aug. 1

Ämnesklassifikation (UKÄ)

  • Lungmedicin och allergi

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