Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Jessica Giacomoni; Mette Habekost; Efrain Cepeda-Prado; Bengt Mattsson; Daniella Rylander Ottosson; Malin Parmar; Janko Kajtez

doi:10.1016/j.xpro.2022.102041

Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Jessica Giacomoni, Mette Habekost, Efrain Cepeda-Prado, Bengt Mattsson, Daniella Rylander Ottosson , Malin Parmar, Janko Kajtez

University of Copenhagen

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.

Originalspråk	engelska
Artikelnummer	102041
Tidskrift	STAR Protocols
Volym	4
Nummer	1
DOI	https://doi.org/10.1016/j.xpro.2022.102041
Status	Published - 2023

Ämnesklassifikation (UKÄ)

Annan medicinteknik

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10.1016/j.xpro.2022.102041Licens: CC BY

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publication in CellPress

NSC-Reconstruct: Novel Strategies for Cell-based Neural Reconstruction
Cattaneo, E. (PI), Parmar, M. (PI), Björklund, T. (PI), Björklund, A. (CoPI), Brüstle, O. (PI), Arenas, E. (PI), Buffo, A. (PI), Barker, R. (PI), Kirkeby, A. (PI), Li, M. (PI), Götz, M. (PI), Vanderhaeghen, P. (PI), Schmandt-Kappel, T. (PI), Strøyer Christophersen, N. (PI), Villaescusa, J. C. (CoPI), Bosio, A. (PI) & Pettersson, P. (Administratör)
European Commission - Horizon 2020
2020/01/01 → 2024/12/31
Projekt: Forskning

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abstract = "Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.",

author = "Jessica Giacomoni and Mette Habekost and Efrain Cepeda-Prado and Bengt Mattsson and {Rylander Ottosson}, Daniella and Malin Parmar and Janko Kajtez",

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AU - Giacomoni, Jessica

AU - Habekost, Mette

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AU - Mattsson, Bengt

AU - Rylander Ottosson , Daniella

AU - Parmar, Malin

AU - Kajtez, Janko

PY - 2023

Y1 - 2023

N2 - Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.

AB - Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.

U2 - 10.1016/j.xpro.2022.102041

DO - 10.1016/j.xpro.2022.102041

M3 - Article

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SN - 2666-1667

VL - 4

JO - STAR Protocols

JF - STAR Protocols

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Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Sammanfattning

Ämnesklassifikation (UKÄ)

Tillgång till dokument

Andra filer och länkar

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Projekt

NSC-Reconstruct: Novel Strategies for Cell-based Neural Reconstruction

Citera det här