Sammanfattning

Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.
Originalspråkengelska
Artikelnummer102041
TidskriftSTAR Protocols
Volym4
Nummer1
DOI
StatusPublished - 2023

Ämnesklassifikation (UKÄ)

  • Annan medicinteknik

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