Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Huy Cuong Tran; Olivier Van Aken

doi:10.1007/978-1-0716-1653-6_1

Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Forskningsoutput: Kapitel i bok/rapport/Conference proceeding › Kapitel samlingsverk › Forskning › Peer review

Sammanfattning

The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.

Originalspråk	engelska
Titel på värdpublikation	Plant Mitochondria
Undertitel på värdpublikation	Methods and Protocols
Redaktörer	Olivier Van Aken, Allan G. Rasmusson
Förlag	Humana Press
Sidor	1-12
Antal sidor	12
ISBN (elektroniskt)	978-1-0716-1653-6
ISBN (tryckt)	978-1-0716-1652-9
DOI	https://doi.org/10.1007/978-1-0716-1653-6_1
Status	Published - 2022

Publikationsserier

Namn	Methods in Molecular Biology
Volym	2363
ISSN (tryckt)	1064-3745
ISSN (elektroniskt)	1940-6029

Bibliografisk information

Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Ämnesklassifikation (UKÄ)

Biokemi och molekylärbiologi
Cellbiologi

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1 Doktorsavhandling (sammanläggning)

Characterization of land plant-specific proteins required for mitochondrial translation initiation
Tran, H. C., 2023 maj 5, Lund University, Faculty of Science. 164 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

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abstract = "The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.",

keywords = "Arabidopsis, Density gradients, Mitochondria, Mitochondrial isolation",

author = "Tran, {Huy Cuong} and {Van Aken}, Olivier",

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doi = "10.1007/978-1-0716-1653-6_1",

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Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients. / Tran, Huy Cuong ; Van Aken, Olivier.

Plant Mitochondria: Methods and Protocols. red. / Olivier Van Aken; Allan G. Rasmusson. Humana Press, 2022. s. 1-12 (Methods in Molecular Biology; Vol. 2363).

Forskningsoutput: Kapitel i bok/rapport/Conference proceeding › Kapitel samlingsverk › Forskning › Peer review

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PY - 2022

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Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

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Forskningsoutput

Characterization of land plant-specific proteins required for mitochondrial translation initiation

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