Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation

Sarote Nitsawang, Rajni Hatti-Kaul, Pawinee Kanasawuda

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. (
Originalspråkengelska
Sidor (från-till)1103-1107
TidskriftEnzyme and Microbial Technology
Volym39
Nummer5
DOI
StatusPublished - 2006

Ämnesklassifikation (UKÄ)

  • Industriell bioteknik

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