Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens

Rune Nilsson, Thomas Brodin, Hans Olov Sjögren

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

An indirect 125I-labeled protein A assay for detection of cell surface-bound rat immunoglobulins is presented. The assay is quantitative and rapid and detects as little as 1 ng of cell surface-bound Ig. It discriminates between antibodies belonging to different IgG subclasses, IgM and IgA. We describe the production and specificity control of the reagents used and show that the test can be used for quantitative analysis. A large number of sera from untreated rats are tested to evaluate the frequency of falsely positive responses and variation due to age, sex and strain of rat. With this test it is relatively easy to quantitate the binding of classes and subclasses of rat immunoglobulins in a small volume (6 μl) of untreated serum.
Originalspråkengelska
Sidor (från-till)179-191
TidskriftJournal of Immunological Methods
Volym55
Utgåva2
DOI
StatusPublished - 1982

Ämnesklassifikation (UKÄ)

  • Immunologi inom det medicinska området

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