Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes

Yi Han Xia, Bao Jian Ding, Shuang Lin Dong, Hong Lei Wang, Per Hofvander, Christer Löfstedt

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Background: Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management. Results: In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr∆11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of C. suppressalis. Both compounds were collected from the transformed N. benthamiana headspace volatiles using solid-phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant. Conclusions: Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies.

Originalspråkengelska
Artikelnummer80
Sidor (från-till)1-18
TidskriftBMC Biology
Volym20
Nummer1
DOI
StatusPublished - 2022 mars 31

Ämnesklassifikation (UKÄ)

  • Biokemi och molekylärbiologi
  • Zoologi

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