RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

Aurelie Baudet; Christine Karlsson; Mehrnaz Safaee Talkhoncheh; Roman Galeev; Mattias Magnusson; Jonas Larsson

doi:10.1182/blood-2012-01-403949

RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

Aurelie Baudet, Christine Karlsson, Mehrnaz Safaee Talkhoncheh, Roman Galeev, Mattias Magnusson, Jonas Larsson

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.

Originalspråk	engelska
Sidor (från-till)	6255-6258
Tidskrift	Blood
Volym	119
Nummer	26
DOI	https://doi.org/10.1182/blood-2012-01-403949
Status	Published - 2012

Ämnesklassifikation (UKÄ)

Hematologi

Tillgång till dokument

10.1182/blood-2012-01-403949

http://www.ncbi.nlm.nih.gov/pubmed/22555972?dopt=Abstract

3 Doktorsavhandling (sammanläggning)

Ex Vivo Expansion of Human Hematopoietic Stem Cells
Safaee Talkhoncheh, M., 2019, Lund: Lund University: Faculty of Medicine. 100 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)
Identification of intrinsic regulators in normal and malignant human hematopoiesis. An RNA-interference approach.
Bidragets översatta titel : Identifiering av genetiska regulatorer i normal and malign human hematopoes: RNA interferensGaleev, R., 2018, Lund: Lund University: Faculty of Medicine. 127 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

Öppen tillgång
Fil
Defining Regulators of Human Hematopoietic Stem Cells
Karlsson, C., 2013, Division of Molecular Medicine and Gene Therapy. 98 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

Citera det här

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title = "RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.",

abstract = "We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.",

author = "Aurelie Baudet and Christine Karlsson and {Safaee Talkhoncheh}, Mehrnaz and Roman Galeev and Mattias Magnusson and Jonas Larsson",

year = "2012",

doi = "10.1182/blood-2012-01-403949",

language = "English",

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pages = "6255--6258",

journal = "Blood",

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publisher = "American Society of Hematology",

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T1 - RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

AU - Baudet, Aurelie

AU - Karlsson, Christine

AU - Safaee Talkhoncheh, Mehrnaz

AU - Galeev, Roman

AU - Magnusson, Mattias

AU - Larsson, Jonas

PY - 2012

Y1 - 2012

N2 - We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.

AB - We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.

U2 - 10.1182/blood-2012-01-403949

DO - 10.1182/blood-2012-01-403949

M3 - Article

C2 - 22555972

SN - 1528-0020

VL - 119

SP - 6255

EP - 6258

JO - Blood

JF - Blood

IS - 26

ER -

RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

Sammanfattning

Ämnesklassifikation (UKÄ)

Tillgång till dokument

Fingeravtryck

Forskningsoutput

Ex Vivo Expansion of Human Hematopoietic Stem Cells

Identification of intrinsic regulators in normal and malignant human hematopoiesis. An RNA-interference approach.

Defining Regulators of Human Hematopoietic Stem Cells

Citera det här