Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

Lars Wallman; Simon Ekström; Mattias Magnusson; Gunnar Bolmsjö; Magnus Olsson; Johan Nilsson; György Marko-Varga; Thomas Laurell

doi:10.1088/0957-0233/17/12/S05

Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

Lars Wallman, Simon Ekström, Mattias Magnusson, Gunnar Bolmsjö, Magnus Olsson, Johan Nilsson, György Marko-Varga, Thomas Laurell

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.

Originalspråk	engelska
Sidor (från-till)	3147-3153
Tidskrift	Measurement Science & Technology
Volym	17
Nummer	12
DOI	https://doi.org/10.1088/0957-0233/17/12/S05
Status	Published - 2006

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Biomedical Engineering (011200011), Robotics (ceased) (LUR000026), Analytical Chemistry (S/LTH) (011001004), Departments at LTH (011200000)

Ämnesklassifikation (UKÄ)

Analytisk kemi
Medicinteknik

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10.1088/0957-0233/17/12/S05

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title = "Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout",

abstract = "A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.",

keywords = "piezodispensing, capillary force, microfluidics, MALDI, mass spectrometry, microchip, robotic, MALDI-TOF MS, automation, protein analysis, proteomics, solid phase extraction",

author = "Lars Wallman and Simon Ekstr{\"o}m and Mattias Magnusson and Gunnar Bolmsj{\"o} and Magnus Olsson and Johan Nilsson and Gy{\"o}rgy Marko-Varga and Thomas Laurell",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Robotics (ceased) (LUR000026), Analytical Chemistry (S/LTH) (011001004), Departments at LTH (011200000)",

year = "2006",

doi = "10.1088/0957-0233/17/12/S05",

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T1 - Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

AU - Wallman, Lars

AU - Ekström, Simon

AU - Magnusson, Mattias

AU - Bolmsjö, Gunnar

AU - Olsson, Magnus

AU - Nilsson, Johan

AU - Marko-Varga, György

AU - Laurell, Thomas

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Robotics (ceased) (LUR000026), Analytical Chemistry (S/LTH) (011001004), Departments at LTH (011200000)

PY - 2006

Y1 - 2006

N2 - A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.

AB - A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.

KW - piezodispensing

KW - capillary force

KW - microfluidics

KW - MALDI

KW - mass spectrometry

KW - microchip

KW - robotic

KW - MALDI-TOF MS

KW - automation

KW - protein analysis

KW - proteomics

KW - solid phase extraction

U2 - 10.1088/0957-0233/17/12/S05

DO - 10.1088/0957-0233/17/12/S05

M3 - Article

SN - 0957-0233

VL - 17

SP - 3147

EP - 3153

JO - Measurement Science & Technology

JF - Measurement Science & Technology

IS - 12

ER -

Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

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Ämnesklassifikation (UKÄ)

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