Separation of 100-kilobase DNA molecules in 10 seconds

O. Bakajin; T. A J Duke; J. Tegenfeldt; C. F. Chou; S. S. Chan; R. H. Austin; E. C. Cox

doi:10.1021/ac015527o

Separation of 100-kilobase DNA molecules in 10 seconds

O. Bakajin, T. A J Duke, J. Tegenfeldt, C. F. Chou, S. S. Chan, R. H. Austin, E. C. Cox

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

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Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

Originalspråk	engelska
Sidor (från-till)	6053-6056
Antal sidor	4
Tidskrift	Analytical Chemistry
Volym	73
Nummer	24
DOI	https://doi.org/10.1021/ac015527o
Status	Published - 2001 dec. 15

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title = "Separation of 100-kilobase DNA molecules in 10 seconds",

abstract = "Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.",

author = "O. Bakajin and Duke, {T. A J} and J. Tegenfeldt and Chou, {C. F.} and Chan, {S. S.} and Austin, {R. H.} and Cox, {E. C.}",

year = "2001",

month = dec,

day = "15",

doi = "10.1021/ac015527o",

language = "English",

volume = "73",

pages = "6053--6056",

journal = "Analytical Chemistry",

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T1 - Separation of 100-kilobase DNA molecules in 10 seconds

AU - Bakajin, O.

AU - Duke, T. A J

AU - Tegenfeldt, J.

AU - Chou, C. F.

AU - Chan, S. S.

AU - Austin, R. H.

AU - Cox, E. C.

PY - 2001/12/15

Y1 - 2001/12/15

N2 - Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

AB - Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

UR - http://www.scopus.com/inward/record.url?scp=0035894044&partnerID=8YFLogxK

U2 - 10.1021/ac015527o

DO - 10.1021/ac015527o

M3 - Article

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SN - 0003-2700

VL - 73

SP - 6053

EP - 6056

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 24

ER -

Separation of 100-kilobase DNA molecules in 10 seconds

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