SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT

Elham Akbari; Jason P. Beech; Pontus Nordenfelt; Jonas Tegenfeldt

SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT

Elham Akbari, Jason P. Beech, Pontus Nordenfelt, Jonas Tegenfeldt

Forskningsoutput: Kapitel i bok/rapport/Conference proceeding › Konferenspaper i proceeding › Peer review

Sammanfattning

Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on cluster size and morphology, using Deterministic Lateral Displacement (DLD). In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.

Originalspråk	engelska
Titel på värdpublikation	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Förlag	Chemical and Biological Microsystems Society
Sidor	1201-1202
Antal sidor	2
ISBN (elektroniskt)	9781733419031
Status	Published - 2021
Evenemang	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, USA Varaktighet: 2021 okt. 10 → 2021 okt. 14

Konferens

Konferens	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Land/Territorium	USA
Ort	Palm Springs, Virtual
Period	2021/10/10 → 2021/10/14

Bibliografisk information

Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

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title = "SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT",

abstract = "Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on cluster size and morphology, using Deterministic Lateral Displacement (DLD). In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.",

keywords = "bacteria clusters, Deterministic Lateral Displacement, group A streptococci, Label-free cell separation",

author = "Elham Akbari and Beech, {Jason P.} and Pontus Nordenfelt and Jonas Tegenfeldt",

note = "Funding Information: This work was carried out within NanoLund at Lund University, device processing was conducted in Lund NanoLab, and bacteria samples were prepared by Nordenfelt group within Department of Clinical Sciences at Lund University. It was funded by the Swedish Research Council (grant number 2019-04102). Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

language = "English",

pages = "1201--1202",

booktitle = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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Akbari, E , Beech, JP , Nordenfelt, P & Tegenfeldt, J 2021, SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT. i MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, s. 1201-1202, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, USA, 2021/10/10.

SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT. / Akbari, Elham ; Beech, Jason P.; Nordenfelt, Pontus et al.

MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. s. 1201-1202.

Forskningsoutput: Kapitel i bok/rapport/Conference proceeding › Konferenspaper i proceeding › Peer review

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T1 - SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT

AU - Akbari, Elham

AU - Beech, Jason P.

AU - Nordenfelt, Pontus

AU - Tegenfeldt, Jonas

N1 - Funding Information: This work was carried out within NanoLund at Lund University, device processing was conducted in Lund NanoLab, and bacteria samples were prepared by Nordenfelt group within Department of Clinical Sciences at Lund University. It was funded by the Swedish Research Council (grant number 2019-04102). Publisher Copyright: © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on cluster size and morphology, using Deterministic Lateral Displacement (DLD). In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.

AB - Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on cluster size and morphology, using Deterministic Lateral Displacement (DLD). In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.

KW - bacteria clusters

KW - Deterministic Lateral Displacement

KW - group A streptococci

KW - Label-free cell separation

M3 - Paper in conference proceeding

AN - SCOPUS:85136922901

SP - 1201

EP - 1202

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT

Sammanfattning

Konferens

Bibliografisk information

Ämnesklassifikation (UKÄ)

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