Single-step partitioning in aqueous two-phase systems

Telma Teixeira Franco; Rajni Hatti-Kaul

doi:10.1385/1-59259-028-4:47

Single-step partitioning in aqueous two-phase systems

Telma Teixeira Franco, Rajni Hatti-Kaul

Bioteknik

Forskningsoutput: Kapitel i bok/rapport/Conference proceeding › Kapitel samlingsverk › Peer review

Sammanfattning

Partitioning in aqueous two-phase system (ATPS) provides a rapid and gentle means of separation of soluble as well as particulate biomaterials, e.g. proteins, nucleic acids, cells, viruses, organelles, and membranes. Partitioning between the two phases is a complex phenomenon, guided mainly by the interaction of the partitioned substance and the phase components, e.g., through hydrogen bonds, van der Waals-, hydrophobic-, and electrostatic- interactions, steric-, and conformational effects, etc.

Originalspråk	engelska
Titel på värdpublikation	Aqueous Two-Phase Systems – Methods and Protocols
Redaktörer	Rajni Hatti-Kaul
Utgivningsort	New Jersey
Förlag	Humana Press
Kapitel	5
Sidor	47-54
ISBN (elektroniskt)	978-1-59259-028-5
ISBN (tryckt)	978-0-89603-541-6, 978-1-61737-067-0
DOI	https://doi.org/10.1385/1-59259-028-4:47
Status	Published - 2000

Ämnesklassifikation (UKÄ)

Industriell bioteknik

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10.1385/1-59259-028-4:47

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abstract = "Partitioning in aqueous two-phase system (ATPS) provides a rapid and gentle means of separation of soluble as well as particulate biomaterials, e.g. proteins, nucleic acids, cells, viruses, organelles, and membranes. Partitioning between the two phases is a complex phenomenon, guided mainly by the interaction of the partitioned substance and the phase components, e.g., through hydrogen bonds, van der Waals-, hydrophobic-, and electrostatic- interactions, steric-, and conformational effects, etc.",

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Single-step partitioning in aqueous two-phase systems

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