STRUCTURAL, FUNCTIONAL AND EVOLUTIONARY STUDIES OF ANTIMICROBIAL PEPTIDES

Mukesh Pasupuleti

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

169 Nedladdningar (Pure)

Sammanfattning

Antimicrobial peptides represent a heterogeneous group that displays multiple modes of action such as bacteriostatic, microbicidal and cytolytic properties that are sequence and concentration dependent. Life threatening infectious disease is now a worldwide crisis and treating them effectively is becoming difficult day by day, due to the emergence of antibiotic resistant strains at alarming rates. Hence, there is an urgent need for new class of antibiotics and, antimicrobial peptides (AMPs) are an ideal candidate for this job. AMPs are gene encoded short (<100 amino acids), amphipathic molecules with hydrophobic and cationic amino acids arranged spatially which exhibit broad-spectrum antimicrobial activity. AMPs form an ancient non-specific type of innate immunity found universally in all living organisms and used as the principal first line of defense against the invading pathogen. AMPs have been in the process of evolution, as have the microbes, for hundreds of years. Despite the long history of co-evolution, AMPs have not lost their ability to kill the microbes totally nor have the microbes learnt to avoid the lethal punch of AMPs. Based upon accumulating positive data, we are encouraged to believe that antimicrobial peptides have a great potential to be the next breakthrough and first novel, truly biological in nature, class of antibiotics.
The purpose of this study was twofold; primarily to elucidate the factors involved in governing the peptide activity and toxicity against membranes, and secondly to design a simple approach where we can boost and spread the spectrum of antimicrobial activity against pathogens such as S. aureus and P. aeruginosa for a peptide that is otherwise non-lethal to the bacteria. Results presented in this thesis show that antimicrobial domains of the anaphylatoxin C3a are structurally and evolutionary conserved. Moreover antimicrobial activity is not governed by a single factor, but instead by a combination of net charge, amphipathicity and helicity. By utilizing a low number of amino acid substitutions at strategic positions in an antimicrobial peptide derived from C3a, CNY20, we were able to develop peptides, which exert a significant activity on both S. aureus and C. albicans in contrast to the parent peptide. Although, antimicrobial activity is not governed by single parameter, the activity can still be boosted by end-tagging of a peptide with hydrophobic oligopeptide stretches. This modification promotes peptide binding to bacteria and subsequent cell wall rupture, but does not increase the toxicity or the protease susceptibility of the peptide. It is noteworthy that end tagging of ultra short peptides spanning 5-7 amino acids with hydrophobic amino acids enhances bactericidal activity, while preserving low toxicity and protease resistance.
Originalspråkengelska
KvalifikationDoktor
Tilldelande institution
  • Dermatologi och venereologi, Lund
Handledare
  • Schmidtchen, Artur, handledare
Tilldelningsdatum2009 sep. 30
Förlag
ISBN (tryckt)978-91-86253-73-8
StatusPublished - 2009

Bibliografisk information

Defence details

Date: 2009-09-30
Time: 09:15
Place: GK-salen, Biomedicinskt Centrum, Sölvegatan 19.

External reviewer(s)

Name: Andersson, Mats
Title: Prof.
Affiliation: Vetenskaplig sekreterare, Karolinska Institutet , Forsknings- och Forskarutbildningsavdelningen, Nobels väg 5, S-171 77 Stockholm, Sweden

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Ämnesklassifikation (UKÄ)

  • Dermatologi och venereologi

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