Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)

Jane Jönsson; Maren Wellenreuther; Torbjörn Tyler

doi:10.1007/s12686-010-9209-2

Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)

Jane Jönsson, Maren Wellenreuther, Torbjörn Tyler

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

Eight novel microsatellite markers were developed for Hieracium s.s. (excluding Pilosella) using a modified FIASCO protocol, and two out of nine microsatellites that were originally developed for Pilosella officinarum were optimised for Hieracium s.s.. All ten microsatellites consistently amplified loci from a wide range of representatives from north and central European species of various sections of the genus. When tested on 19 individuals, nine microsatellites were found to be highly polymorphic with 6–29 alleles, while one microsatellite marker was monomorphic. Cross-amplification showed that six of the ten microsatellites also amplified products in one taxon of Pilosella, P. floribunda × officinarum.

Originalspråk	engelska
Sidor (från-till)	295-300
Tidskrift	Conservation Genetics Resources
Volym	2
Nummer	1
DOI	https://doi.org/10.1007/s12686-010-9209-2
Status	Published - 2010

Ämnesklassifikation (UKÄ)

Biologiska vetenskaper
Botanik

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10.1007/s12686-010-9209-2

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title = "Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)",

abstract = "Eight novel microsatellite markers were developed for Hieracium s.s. (excluding Pilosella) using a modified FIASCO protocol, and two out of nine microsatellites that were originally developed for Pilosella officinarum were optimised for Hieracium s.s.. All ten microsatellites consistently amplified loci from a wide range of representatives from north and central European species of various sections of the genus. When tested on 19 individuals, nine microsatellites were found to be highly polymorphic with 6–29 alleles, while one microsatellite marker was monomorphic. Cross-amplification showed that six of the ten microsatellites also amplified products in one taxon of Pilosella, P. floribunda × officinarum.",

keywords = "microsatellite marker, FIASCO, Hieracium, Pilosella, genetic variation, cross-amplification",

author = "Jane J{\"o}nsson and Maren Wellenreuther and Torbj{\"o}rn Tyler",

year = "2010",

doi = "10.1007/s12686-010-9209-2",

language = "English",

volume = "2",

pages = "295--300",

journal = "Conservation Genetics Resources",

issn = "1877-7260",

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TY - JOUR

T1 - Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)

AU - Jönsson, Jane

AU - Wellenreuther, Maren

AU - Tyler, Torbjörn

PY - 2010

Y1 - 2010

N2 - Eight novel microsatellite markers were developed for Hieracium s.s. (excluding Pilosella) using a modified FIASCO protocol, and two out of nine microsatellites that were originally developed for Pilosella officinarum were optimised for Hieracium s.s.. All ten microsatellites consistently amplified loci from a wide range of representatives from north and central European species of various sections of the genus. When tested on 19 individuals, nine microsatellites were found to be highly polymorphic with 6–29 alleles, while one microsatellite marker was monomorphic. Cross-amplification showed that six of the ten microsatellites also amplified products in one taxon of Pilosella, P. floribunda × officinarum.

AB - Eight novel microsatellite markers were developed for Hieracium s.s. (excluding Pilosella) using a modified FIASCO protocol, and two out of nine microsatellites that were originally developed for Pilosella officinarum were optimised for Hieracium s.s.. All ten microsatellites consistently amplified loci from a wide range of representatives from north and central European species of various sections of the genus. When tested on 19 individuals, nine microsatellites were found to be highly polymorphic with 6–29 alleles, while one microsatellite marker was monomorphic. Cross-amplification showed that six of the ten microsatellites also amplified products in one taxon of Pilosella, P. floribunda × officinarum.

KW - microsatellite marker

KW - FIASCO

KW - Hieracium

KW - Pilosella

KW - genetic variation

KW - cross-amplification

U2 - 10.1007/s12686-010-9209-2

DO - 10.1007/s12686-010-9209-2

M3 - Article

SN - 1877-7260

VL - 2

SP - 295

EP - 300

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 1

ER -

Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)

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