The new cell irradiation facility at the Lund nuclear probe

Natalia Arteaga; Jan Pallon; Magnus Gram; Vaida Auzelyte; Mikael Elfman; Per Kristiansson; Klas Malmqvist; Christer Nilsson; Marie Wegdén

doi:10.1016/j.nimb.2007.01.255

The new cell irradiation facility at the Lund nuclear probe

Natalia Arteaga, Jan Pallon, Magnus Gram, Vaida Auzelyte, Mikael Elfman, Per Kristiansson, Klas Malmqvist, Christer Nilsson, Marie Wegdén

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

The CELLION project is directed towards the studies on cellular response to targeted single ions. This paper gives an account of the modifications made at the Lund sub-micron beam line in order to create a Single Ion Hit Facility for biological applications within the CELLION project. The most relevant new feature is the specially developed software used to locate the cells. A program for cell recognition and localization that gives the coordinates of the centre of the cells has been designed. The recognition is made online. A picture taken by a microscope is used as input parameter for the recognition program. Using V79 Chinese hamster cells, the recognition procedure can be done in less than 0.5 s for a picture size of 800 x 600 pixels with approximately 96% efficiency. This paper also reports target accuracy test results and the first non-targeted irradiation procedure performed at the Lund sub-micron beam line. (c) 2007 Elsevier B.V. All rights reserved.

Originalspråk	engelska
Sidor (från-till)	91-96
Tidskrift	Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
Volym	260
Nummer	1
DOI	https://doi.org/10.1016/j.nimb.2007.01.255
Status	Published - 2007
Evenemang	10th International Conference on Nuclear Microprobe Technology and Applications and 2nd International Workshop on Proton Beam Writing - Singapore, Singapore Varaktighet: 2006 juli 9 → 2006 juli 14

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007), Division of Infection Medicine (BMC) (013024020)

Ämnesklassifikation (UKÄ)

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10.1016/j.nimb.2007.01.255

1 Doktorsavhandling (sammanläggning)

Single-Proton Irradiation of Living Cells - Development of New Tools for Low-Dose Radiation Research
Arteaga, N., 2010, Department of Physics, Lund University. 133 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

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title = "The new cell irradiation facility at the Lund nuclear probe",

abstract = "The CELLION project is directed towards the studies on cellular response to targeted single ions. This paper gives an account of the modifications made at the Lund sub-micron beam line in order to create a Single Ion Hit Facility for biological applications within the CELLION project. The most relevant new feature is the specially developed software used to locate the cells. A program for cell recognition and localization that gives the coordinates of the centre of the cells has been designed. The recognition is made online. A picture taken by a microscope is used as input parameter for the recognition program. Using V79 Chinese hamster cells, the recognition procedure can be done in less than 0.5 s for a picture size of 800 x 600 pixels with approximately 96% efficiency. This paper also reports target accuracy test results and the first non-targeted irradiation procedure performed at the Lund sub-micron beam line. (c) 2007 Elsevier B.V. All rights reserved.",

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author = "Natalia Arteaga and Jan Pallon and Magnus Gram and Vaida Auzelyte and Mikael Elfman and Per Kristiansson and Klas Malmqvist and Christer Nilsson and Marie Wegd{\'e}n",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007), Division of Infection Medicine (BMC) (013024020); 10th International Conference on Nuclear Microprobe Technology and Applications and 2nd International Workshop on Proton Beam Writing ; Conference date: 09-07-2006 Through 14-07-2006",

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language = "English",

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AU - Gram, Magnus

AU - Auzelyte, Vaida

AU - Elfman, Mikael

AU - Kristiansson, Per

AU - Malmqvist, Klas

AU - Nilsson, Christer

AU - Wegdén, Marie

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007), Division of Infection Medicine (BMC) (013024020)

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AB - The CELLION project is directed towards the studies on cellular response to targeted single ions. This paper gives an account of the modifications made at the Lund sub-micron beam line in order to create a Single Ion Hit Facility for biological applications within the CELLION project. The most relevant new feature is the specially developed software used to locate the cells. A program for cell recognition and localization that gives the coordinates of the centre of the cells has been designed. The recognition is made online. A picture taken by a microscope is used as input parameter for the recognition program. Using V79 Chinese hamster cells, the recognition procedure can be done in less than 0.5 s for a picture size of 800 x 600 pixels with approximately 96% efficiency. This paper also reports target accuracy test results and the first non-targeted irradiation procedure performed at the Lund sub-micron beam line. (c) 2007 Elsevier B.V. All rights reserved.

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JO - Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms

JF - Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms

IS - 1

T2 - 10th International Conference on Nuclear Microprobe Technology and Applications and 2nd International Workshop on Proton Beam Writing

Y2 - 9 July 2006 through 14 July 2006

ER -

The new cell irradiation facility at the Lund nuclear probe

Sammanfattning

Bibliografisk information

Ämnesklassifikation (UKÄ)

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Forskningsoutput

Single-Proton Irradiation of Living Cells - Development of New Tools for Low-Dose Radiation Research

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