Towards molecular-imprint based SPE of local anaesthetics

LI Andersson; M Abdel-Rehim; L Nicklasson; Leif Schweitz; Staffan Nilsson

doi:10.1007/BF02493355

Towards molecular-imprint based SPE of local anaesthetics

LI Andersson, M Abdel-Rehim, L Nicklasson, Leif Schweitz, Staffan Nilsson

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Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.

Originalspråk	engelska
Sidor (från-till)	S65-S69
Tidskrift	Chromatographia
Volym	55
DOI	https://doi.org/10.1007/BF02493355
Status	Published - 2002

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Ämnesklassifikation (UKÄ)

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10.1007/BF02493355

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title = "Towards molecular-imprint based SPE of local anaesthetics",

abstract = "The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.",

keywords = "anaesthetics, solid-phase extraction, molecular imprinting",

author = "LI Andersson and M Abdel-Rehim and L Nicklasson and Leif Schweitz and Staffan Nilsson",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)",

year = "2002",

doi = "10.1007/BF02493355",

language = "English",

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journal = "Chromatographia",

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T1 - Towards molecular-imprint based SPE of local anaesthetics

AU - Andersson, LI

AU - Abdel-Rehim, M

AU - Nicklasson, L

AU - Schweitz, Leif

AU - Nilsson, Staffan

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

PY - 2002

Y1 - 2002

N2 - The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.

AB - The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.

KW - anaesthetics

KW - solid-phase extraction

KW - molecular imprinting

U2 - 10.1007/BF02493355

DO - 10.1007/BF02493355

M3 - Article

SN - 0009-5893

VL - 55

SP - S65-S69

JO - Chromatographia

JF - Chromatographia

ER -

Towards molecular-imprint based SPE of local anaesthetics

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