TY - JOUR
T1 - Vascular effects of helodermin, helospectin I and helospectin II
T2 - a comparison with vasoactive intestinal peptide (VIP)
AU - Grundemar, L
AU - Högestätt, E D
PY - 1990/3
Y1 - 1990/3
N2 - 1. Helodermin, helospectin I and helospectin II, peptides recently isolated from the salivary gland venom of Heloderma suspectum, were compared to vasoactive intestinal peptide (VIP) with respect to effects on systemic blood pressure and on isolated femoral arteries in the rat. 2. They all reduced blood pressure in a dose-dependent manner; helodermin was less effective than VIP. However, at doses higher than 1 nmol kg-1 all four peptides reduced blood pressure to about the same extent. 3. The half-life of the hypotensive effect of VIP was longer than that of helodermin and the helospectins. 4. VIP and helodermin were equally potent in relaxing femoral arteries precontracted with phenylephrine or prostaglandin F2 alpha. 5. Helospectin I and II relaxed phenylephrine-contracted vessels to the same extent as VIP but with a lower potency. 6. Addition of VIP 1 microM to preparations exposed to helodermin 1 microM or to either of the helospectins did not produce a further relaxation. 7. The findings indicate that VIP, helodermin and helospectin I and II have a similar profile of action and therefore may act on a common receptor.
AB - 1. Helodermin, helospectin I and helospectin II, peptides recently isolated from the salivary gland venom of Heloderma suspectum, were compared to vasoactive intestinal peptide (VIP) with respect to effects on systemic blood pressure and on isolated femoral arteries in the rat. 2. They all reduced blood pressure in a dose-dependent manner; helodermin was less effective than VIP. However, at doses higher than 1 nmol kg-1 all four peptides reduced blood pressure to about the same extent. 3. The half-life of the hypotensive effect of VIP was longer than that of helodermin and the helospectins. 4. VIP and helodermin were equally potent in relaxing femoral arteries precontracted with phenylephrine or prostaglandin F2 alpha. 5. Helospectin I and II relaxed phenylephrine-contracted vessels to the same extent as VIP but with a lower potency. 6. Addition of VIP 1 microM to preparations exposed to helodermin 1 microM or to either of the helospectins did not produce a further relaxation. 7. The findings indicate that VIP, helodermin and helospectin I and II have a similar profile of action and therefore may act on a common receptor.
KW - Amino Acid Sequence
KW - Animals
KW - Blood Pressure/drug effects
KW - Dinoprost/pharmacology
KW - Female
KW - Half-Life
KW - Hemodynamics/drug effects
KW - In Vitro Techniques
KW - Male
KW - Molecular Sequence Data
KW - Peptides/pharmacology
KW - Phenylephrine/pharmacology
KW - Rats
KW - Rats, Inbred Strains
KW - Vasoactive Intestinal Peptide/pharmacology
U2 - 10.1111/j.1476-5381.1990.tb12962.x
DO - 10.1111/j.1476-5381.1990.tb12962.x
M3 - Article
C2 - 2331581
SN - 0007-1188
VL - 99
SP - 526
EP - 528
JO - British Journal of Pharmacology
JF - British Journal of Pharmacology
IS - 3
ER -