TY - JOUR
T1 - Yellow and Orange Fluorescent Proteins with Tryptophan-based Chromophores
AU - Bozhanova, Nina G.
AU - Baranov, Mikhail S.
AU - Sarkisyan, Karen S.
AU - Gritcenko, Roman
AU - Mineev, Konstantin S.
AU - Golodukhina, Svetlana V.
AU - Baleeva, Nadezhda S.
AU - Lukyanov, Konstantin A.
AU - Mishin, Alexander S.
PY - 2017/7/21
Y1 - 2017/7/21
N2 - Rapid development of new microscopy techniques exposed the need for genetically encoded fluorescent tags with special properties. Recent works demonstrated the potential of fluorescent proteins with tryptophan-based chromophores. We applied rational design and random mutagenesis to the monomeric red fluorescent protein FusionRed and found two groups of mutants carrying a tryptophan-based chromophore: with yellow (535 nm) or orange (565 nm) emission. On the basis of the properties of proteins, a model synthetic chromophore, and a computational modeling, we concluded that the presence of a ketone-containing chromophore in different isomeric forms can explain the observed yellow and orange phenotypes.
AB - Rapid development of new microscopy techniques exposed the need for genetically encoded fluorescent tags with special properties. Recent works demonstrated the potential of fluorescent proteins with tryptophan-based chromophores. We applied rational design and random mutagenesis to the monomeric red fluorescent protein FusionRed and found two groups of mutants carrying a tryptophan-based chromophore: with yellow (535 nm) or orange (565 nm) emission. On the basis of the properties of proteins, a model synthetic chromophore, and a computational modeling, we concluded that the presence of a ketone-containing chromophore in different isomeric forms can explain the observed yellow and orange phenotypes.
U2 - 10.1021/acschembio.7b00337
DO - 10.1021/acschembio.7b00337
M3 - Article
C2 - 28525263
AN - SCOPUS:85025170752
SN - 1554-8929
VL - 12
SP - 1867
EP - 1873
JO - ACS Chemical Biology
JF - ACS Chemical Biology
IS - 7
ER -