Yersinia enterocolitica-mediated degradation of neutrophil extracellular traps (NETs)

Helene Möllerherm, Ariane Neumann, Katrin Schilcher, Stefanie Blodkamp, Nathalie E Zeitouni, Petra Dersch, Petra Lüthje, Hassan Y. Naim, Annelies S Zinkernagel, Maren von Köckritz-Blickwede

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review


Neutrophil extracellular trap (NET) formation is described as a tool of the innate host defence to fight against invading pathogens. Fibre-like DNA structures associated with proteins such as histones, cell-specific enzymes and antimicrobial peptides are released, thereby entrapping invading pathogens. It has been reported that several bacteria are able to degrade NETs by nucleases and thus evade the NET-mediated entrapment. Here we studied the ability of three different Yersinia serotypes to induce and degrade NETs. We found that the common Yersinia enterocolitica serotypes O:3, O:8 and O:9 were able to induce NETs in human blood-derived neutrophils during the first hour of co-incubation. At later time points, the NET amount was reduced, suggesting that degradation of NETs has occurred. This was confirmed by NET degradation assays with phorbol-myristate-acetate-pre-stimulated neutrophils. In addition, we found that the Yersinia supernatants were able to degrade purified plasmid DNA. The absence of Ca(2+) and Mg(2+) ions, but not that of a protease inhibitor cocktail, completely abolished NET degradation. We therefore postulate that Y. enterocolitica produces Ca(2+)/Mg(2+)-dependent NET-degrading nucleases as shown for some Gram-positive pathogens.

TidskriftFEMS Microbiology Letters
StatusPublished - 2015 dec.
Externt publiceradJa


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